MAB’Solut, your expert in monoclonal antibodies
MAB’Solut, key experienced actor of more than 15 years in the fields of therapeutic, diagnostic and theranostic antibodies, is specialized in customized antibodies for Research and Development.
This comprehensive and global offer covers all the stages from the antigen definition to the in vitro and in vivo efficacy and toxicity studies in small animals, through the steps of production & purification and antibodies engineering modifications.
Located in the heart of the Genopole Biopark in Evry (Paris Area), MAB’Solut provides a wide range of high added value and customized services dedicated to complete successfully your projects. MAB’Solut is described as a reliable partner for a large number of professionals from academic research to the biotech/pharmaceutical industrials and diagnostic companies.
(1) We control every step of production and optimization processes for monoclonal antibodies or recombinant proteins such as:
- Design, optimization and preparation of antigen (hapten coupling, fragmentation …),
- Immunizations, fusions, screening, selection and production of hybridomas,
- Production from hybridoma and/or mammalian cells (CHO or HEK293 cells transfected, steady or transiently state)
- Production scale from 1mg to 100g using shake flask, Cell lines, roller bottles, hollow fibers, wave and disposable 50-liters stirred-bioreactors
- Purification by FPLC (Akta basic, Purifier, Explorer and Avant) using A, G, L protein affinity columns, preparative size exclusion chromatography and desalting columns in endotoxin free conditions,
- Characterization and in-vitro validation: Western Blot, capillary electrophoresis in reduced or unreduced conditions, ELISA simplex or multiplex, flow cytometry, FPLC / HPLC gel filtration, endotoxin detection, thermal stability and bioassays (differentiation, PBMC activation, proliferation, apoptosis, ADCC, CDC, phagocytosis …),
(2) Our services offer you our deep experience dedicated to core-antibody modifications by molecular biology and/or chemical engineering:
- Development and validation of immunoassays and bioassays,
- Molecular biology and engineering for the development of specific antibodies: binding optimization, chimerization, humanization, fragments (scFv, ScFV2, dAb), bispecific antibody, mutants library construction,
- Chemical Fragmentation (Fab, Fab’2) and antibodies coupling (biotin, enzymes, fluorophores, toxic molecules …)
(3) MAB’Solut covers all the steps related to in-vivo studies & therapeutic antibody-based projects due to a certified animal house facility at Genopole site on small animals (including transgenic animals) for any type of molecule:
- Efficacy, especially in oncology (syngenic, metastatic and xenograft models) and in ophthalmology (rat corneal neovascularization model),
- Pharmacology / biodistribution (PK, PD),
- Toxicology (pathology, histocytopathology …),
- Development of specific in vivo models.
Moreover, the company has a recognized and high level of expertise in the creation of antibodies targeting a diverse range of biological and chemical antigens, low or non-immunogenic, and in particular small molecules (less than 600 Dalton).
Key reasons to work with MAB’Solut:
- To be assured of the quality and relevance of the service and products provided
- To have a single point of contact, dedicated and specialized listening to customer needs and providing it with effective communication
- To arrange customized solutions and appropriate strategies for each project
- To be assured of responsiveness and compliance with defined deadlines
- To have the entire intellectual property of antibodies, hybridomas, immunoassays or other products or processes developed
Please do not hesitate to contact us regarding your projects and specific requests.